A research team led by Oak Ridge National Laboratory bioengineered a microbe to efficiently turn waste into itaconic acid, an industrial chemical used in plastics and paints.
Producing itaconic acid currently involves fungi feeding on relatively pure sugars, which can be expensive. In ORNL’s demonstration, the team used lignin, a waste product from biorefineries and paper mills, to grow the bacterium Pseudomonas putida for potentially cheaper itaconic production.
Scientists genetically engineered bacteria for itaconic acid production, creating dynamic controls that separate microbial growth and production phases for increased efficiency and acid yield. Credit: NREL
The trick was to separate the microbes’ growth phase from itaconic production using dynamic controls. ORNL designed and deployed a biosensor that triggers the metabolic pathway for itaconic acid production only after the microbes consume all the nitrogen that fuels their growth.
“This technology could provide additional revenue for biorefineries by turning lignin into a high-value chemical,” ORNL’s Adam Guss said. “One strain achieved nearly 90% of theoretical yield during the production phase and could be further optimized. We can also apply these methods to a range of carbon waste streams.”
Source: ORNL
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