Puberty triggers brain rewiring in genetic condition tied to autism, mouse study suggests

Changes in brain connectivity before and after puberty may explain why some children with a rare genetic disorder have a higher risk of developing autism or schizophrenia, according to a UCLA Health study.

Developmental psychiatric disorders like autism and schizophrenia are associated with changes in brain functional connectivity. However, the complexity of these conditions make it difficult to understand the underlying biological causes. By studying genetically defined brain disorders, researchers at UCLA Health and collaborators have shed light on possible mechanisms.

The UCLA study examined a particular genetic condition called chromosome 22q11.2 deletion syndrome—caused by missing DNA on chromosome 22—which is associated with a higher risk of developing neuropsychiatric conditions such as autism and schizophrenia. But the underlying biological basis of this association has not been well understood.

In the study published in the journal Science Advances, researchers used functional brain imaging in both mice and humans to investigate potential mechanisms driving the connection between the genetic mutation and development of neuropsychiatric conditions.

Functional brain imaging showed the brain regions in both humans and genetically modified mice were hyperconnected before puberty before switching to being under-connected after puberty, particularly in brain regions tied to social skills and autism.

Co-senior author Carrie Bearden, Professor at the UCLA Health Semel Institute and the UCLA Brain Research Institute, said changes at the synapse level appear to explain the sudden shift in connectivity and associated effects on social behavior.

“Differences in functional connectivity observed on MRI are commonly found in psychiatric disorders, but we don’t have a good understanding of why. It was really valuable to study this phenomenon across species,” Bearden said.

Using mice that were genetically modified to mimic chromosome 22q11.2 deletion syndrome, Bearden and her colleagues at the Italian Institute of Technology found younger mice had a larger density of dendritic spines—small protrusions on brain cells used to communicate with other neurons via synapses—during childhood compared to wild-type (normal) mice.

After the mice reached the equivalent of puberty, the number of dendritic spines sharply decreased compared to wild-type mice.

The protein GSK3-beta, which is involved in synapse regulation, may play a role in the connectivity changes. Bearden and her collaborators used a drug to inhibit GSK3-beta, which worked to temporarily restore brain activity and dendritic spine density in the mice, possibly by regulating the removal of dendritic spines.

Brain imaging of humans with the condition also found the brain regions affected by the connectivity changes had enriched genes related to GSK3-beta. Further, the brain connectivity changes were related to social behavior in humans, suggesting that the altered wiring contributes to autism traits.

These findings, Bearden said, suggest that synaptic dysfunction drives the changes in brain activity and could be a target to prevent or reduce symptoms caused by chromosome 22q11.2 deletion syndrome.

“These findings strongly suggest that over-weeding of synapses during development may contribute to the behavioral challenges we see,” Bearden said.

Changes in brain connectivity before and after puberty may explain why some children with a rare genetic disorder have a higher risk of developing autism or schizophrenia, according to a UCLA Health study.

Developmental psychiatric disorders like autism and schizophrenia are associated with changes in brain functional connectivity. However, the complexity of these conditions make it difficult to understand the underlying biological causes. By studying genetically defined brain disorders, researchers at UCLA Health and collaborators have shed light on possible mechanisms.

The UCLA study examined a particular genetic condition called chromosome 22q11.2 deletion syndrome—caused by missing DNA on chromosome 22—which is associated with a higher risk of developing neuropsychiatric conditions such as autism and schizophrenia. But the underlying biological basis of this association has not been well understood.

In the study published in the journal Science Advances, researchers used functional brain imaging in both mice and humans to investigate potential mechanisms driving the connection between the genetic mutation and development of neuropsychiatric conditions.

Functional brain imaging showed the brain regions in both humans and genetically modified mice were hyperconnected before puberty before switching to being under-connected after puberty, particularly in brain regions tied to social skills and autism.

Co-senior author Carrie Bearden, Professor at the UCLA Health Semel Institute and the UCLA Brain Research Institute, said changes at the synapse level appear to explain the sudden shift in connectivity and associated effects on social behavior.

“Differences in functional connectivity observed on MRI are commonly found in psychiatric disorders, but we don’t have a good understanding of why. It was really valuable to study this phenomenon across species,” Bearden said.

Using mice that were genetically modified to mimic chromosome 22q11.2 deletion syndrome, Bearden and her colleagues at the Italian Institute of Technology found younger mice had a larger density of dendritic spines—small protrusions on brain cells used to communicate with other neurons via synapses—during childhood compared to wild-type (normal) mice.

After the mice reached the equivalent of puberty, the number of dendritic spines sharply decreased compared to wild-type mice.

The protein GSK3-beta, which is involved in synapse regulation, may play a role in the connectivity changes. Bearden and her collaborators used a drug to inhibit GSK3-beta, which worked to temporarily restore brain activity and dendritic spine density in the mice, possibly by regulating the removal of dendritic spines.

Brain imaging of humans with the condition also found the brain regions affected by the connectivity changes had enriched genes related to GSK3-beta. Further, the brain connectivity changes were related to social behavior in humans, suggesting that the altered wiring contributes to autism traits.

These findings, Bearden said, suggest that synaptic dysfunction drives the changes in brain activity and could be a target to prevent or reduce symptoms caused by chromosome 22q11.2 deletion syndrome.

“These findings strongly suggest that over-weeding of synapses during development may contribute to the behavioral challenges we see,” Bearden said.